LEF1 influences diabetic retinopathy and retinal pigment epithelial cell ferroptosis via the miR-495-3p/GRP78 axis through lnc-MGC

Figure 5 lnc-MGC affects the progression of diabetic retinopathy through miR-495-3p. A: Transfection efficiency was detected by RT-qPCR; B: Cell proliferation was detected by CCK-8 assay; C: Cell apoptosis was detected by TUNEL assay. The scale bar represents 100 μm; D: The expression of the inflammatory cytokines TNF-α, IL-1β, IL-6 was detected by ELISA; E: reactive oxygen species were detected by flow cytometry; F-H: Western blotting was used to detect the expression of related proteins. ^aP < 0.05, ^bP < 0.01, and ^cP < 0.001. Inhibitor: MiR-495-3p inhibitor; NC: Normal control; HG: High glucose; ROS: Reactive oxygen species.