LEF1 influences diabetic retinopathy and retinal pigment epithelial cell ferroptosis via the miR-495-3p/GRP78 axis through lnc-MGC

Figure 2 Inhibition of lnc-MGC protects retinal pigment epithelial cells. A: RT-qPCR was used to detect the transfection efficiency; B: CCK-8 was used to detect cell proliferation activity; C: TUNEL was used to detect cell apoptosis. The scale bar represents 100 μm; D: ELISA was used to detect the expression of the inflammatory factors TNF-α, IL-6 and IL-1β; E: Flow cytometry was used to detect reactive oxygen species; F: Western blotting was used to detect the expression of the apoptosis-related proteins Bax, Bcl-2 and cleaved-caspase 3. ^aP < 0.05, ^bP < 0.01, and ^cP < 0.001. NC: Normal control; HG: High glucose; ROS: Reactive oxygen species.