N6-methyladenosine methyltransferase Wilms tumor 1-associated protein impedes diabetic wound healing through epigenetically activating DNA methyltransferase 1

Figure 5 Wilms tumor 1-associated protein-DNA methyltransferase 1 axis regulates human umbilical vein endothelial cell function. Human umbilical vein endothelial cells (HUVECs) were stimulated with high glucose and treated with siWilms tumor 1-associated protein and DNA methyltransferase 1 (DNMT1) overexpression vectors. A: The protein level of DNMT1 in the dorsal skin of mice was detected by western blotting; B: Cell viability was detected by cell counting kit 8 assay; C: The angiogenesis ability of HUVECs was measured by tube formation assay; D: Cell migration was analyzed by Transwell assay. ^cP < 0.001; HG: High glucose; WTAP: Wilms tumor 1-associated protein; DNMT1: DNA methyltransferase 1; m6A: N6-methyladenosine; NC: Negative controls.