N6-methyladenosine methyltransferase Wilms tumor 1-associated protein impedes diabetic wound healing through epigenetically activating DNA methyltransferase 1

Figure 4 Wilms tumor 1-associated protein modulates the N6-methyladenosine modification of DNA methyltransferase 1. A: The N6-methyladenosine (m6A)-modified motif on DNA methyltransferase 1 (DNMT1) mRNA towards Wilms tumor 1-associated protein (WTAP) was predicted; B: A sequence-based m6A modification site predictor SRAMP identified the m6A modification site on DNMT1 mRNA; C: MeRIP-PCR analysis was performed to measure the m6A enrichment in human umbilical vein endothelial cells treated with siNC or siWTAP; D: The stability of DNMT1 mRNA was analyzed by actinomycin D assay in human umbilical vein endothelial cells treated with siNC or siWTAP. ^bP < 0.01; ^cP < 0.001; WTAP: Wilms tumor 1-associated protein; DNMT1: DNA methyltransferase 1; m6A: N6-methyladenosine; NC: Negative controls.