Plantamajoside improves type 2 diabetes mellitus pancreatic β-cell damage by inhibiting endoplasmic reticulum stress through Dnajc1 up-regulation

Figure 6 Plantamajoside downregulates the expression of apoptosis and endoplasmic reticulum stress-related factors in pancreatic tissue of type 2 diabetes mellitus mice and upregulates Dnajc1 expression. A and B: Impact of plantamajoside (PMS) on apoptosis and the expression of endoplasmic reticulum stress-related proteins in the pancreatic tissue of type 2 diabetes mellitus (T2DM) mice was evaluated using TUNEL staining and Western Blot. After PMS intervention, the TUNEL-positive area in the pancreatic tissue of T2DM mice decreased; C-F: Western blot results showing that expression levels of Bcl-2 increased, while expression levels of Bax and CytC decreased after PMS intervention; G-L: Western blot results showing that expressions of ATF6, XBP1, Ddit3 (CHOP), and GRP78 were downregulated, and expression of Dnajc1 was upregulated after PMS intervention. n = 3 per group. ^bP < 0.01 vs the normal control group. ^cP < 0.05, ^dP < 0.01 vs the T2DM group. PMS-H: Plantamajoside high-dose group.