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©The Author(s) 2025.
World J Diabetes. Feb 15, 2025; 16(2): 99053
Published online Feb 15, 2025. doi: 10.4239/wjd.v16.i2.99053
Published online Feb 15, 2025. doi: 10.4239/wjd.v16.i2.99053
Figure 4 Protective effects of plantamajoside on high glucose + palmitic acid -induced pancreatic β-cell damage are attenuated after Dnajc1 silencing.
A: Cell viability was assessed using MTT assay. Dnajc1 was silenced using a transfection reagent, and the cells were divided into five groups: Normal control (NC) group, high glucose (HG) + palmitic acid (PA) group, plantamajoside (PMS) high-dose group (PMS-H) + HG + PA group, si-Dnajc1 + HG + PA group, and si-Dnajc1 + PMS-H + HG + PA group; B-D: The protective effect of PMS on HG + PA-induced pancreatic β-cell damage was weakened after Dnajc1 silencing. Silencing Dnajc1 weakened or eliminated the effects of PMS on insulin levels B), lactate dehydrogenase activity in the supernatant, and reactive oxygen species levels within the cells; E-I: Silencing Dnajc1 weakened or eliminated the impact of PMS intervention on the apoptosis rate and the expression of apoptosis-related proteins (Bcl-2, Bax, and CytC) in HG + PA-induced MIN6 cells; J-O: The regulatory effect of PMS on endoplasmic reticulum stress-related proteins [ATF6, XBP1, Ddit3 (CHOP), GRP78, Dnajc1] disappeared after silencing Dnajc1. A-D: n = 6 per group; E-O: n = 3 per group. aP < 0.05, bP < 0.01 vs the NC group; cP < 0.05, dP < 0.01 vs the HG + PA group.
- Citation: Wang D, Wang YS, Zhao HM, Lu P, Li M, Li W, Cui HT, Zhang ZY, Lv SQ. Plantamajoside improves type 2 diabetes mellitus pancreatic β-cell damage by inhibiting endoplasmic reticulum stress through Dnajc1 up-regulation. World J Diabetes 2025; 16(2): 99053
- URL: https://www.wjgnet.com/1948-9358/full/v16/i2/99053.htm
- DOI: https://dx.doi.org/10.4239/wjd.v16.i2.99053