Plantamajoside improves type 2 diabetes mellitus pancreatic β-cell damage by inhibiting endoplasmic reticulum stress through Dnajc1 up-regulation

Figure 1 Plantamajoside intervention improves high glucose + palmitic acid-induced injury in pancreatic β-cells. A: MIN6 cells were first treated with plantamajoside (PMS) and then subjected to MTT assay to measure cell viability; B: Subsequently, cells were treated with 40 mmol/L high glucose + 0.4 mmol/L palmitic acid, and concurrently treated with different concentrations of PMS. MTT results indicate that 12.5 μM, 25 μM, and 50 μM PMS did not significantly affect MIN6 cell viability; C: ELISA results showing that PMS intervention increases insulin (INS) levels in the supernatant; D: PMS intervention decreases the activity of lactate dehydrogenase (LDH); E: In the supernatant and reactive oxygen species (ROS) levels within the cells. Data are presented as mean ± SD, n = 6 for A-D. ^aP < 0.05, ^bP < 0.01 vs the normal control group; ^cP < 0.05, ^dP < 0.01 vs the high glucose + palmitic acid group.