Electroacupuncture alleviates diabetic peripheral neuropathy through modulating mitochondrial biogenesis and suppressing oxidative stress

Figure 3 Relevant indexes of peripheral nerve function of rats in different groups. A and B: Weekly changes of withdrawal threshold (A) and latency (B); C and D: Changes before and after intervention of withdrawal threshold (C) and latency (D); E and F: Representative immunofluorescence images of nerve fiber in hind paw skin of rats and (E) measurement of intraepidermal nerve fiber density (IENFD) (F); G-I: The dotted line indicates the dermal-epidermal junction, and the arrow refers to the nerve fiber endings passing through the dermal-epidermal junction, which is used for nerve fiber counting. Levels of motor nerve conduction velocity (MNCV) (G), lower limb skin blood flow (H), and sciatic nerve blood flow (I) of rats in each group after intervention (n = 3-5). ^aP < 0.05, ^bP < 0.01 model group vs control group; ^cP < 0.05, ^dP < 0.01 electroacupuncture (EA) group vs model group; ^eP < 0.05, ^fP < 0.01 EA + EX527 group vs EA group; ^gP < 0.05, ^hP < 0.01 post-intervention vs pre-intervention.