Transcriptome and single-cell profiling of the mechanism of diabetic kidney disease

Figure 6 Diabetic kidney disease mostly occurred during the mid-differentiation stage of endothelial cells. A: Expression levels of caspase 1 (CASP1) and sirtuin 2 (SIRT2) in each cell cluster; B: Expression of CASP1 and SIRT2 in each group; C: Pseudo-temporal analysis reveals 11 states in the differentiation process of endothelial cells; D: Pseudo-temporal analysis reveals that diabetic kidney disease mostly occurs during the mid-differentiation stage of endothelial cells. DKD: Diabetic kidney disease; SIRT2: Sirtuin 2; CASP1: Caspase 1; ENDO: Endothelial cells; TAL: Thick ascending limb; PT_VACM1: Proximal tubule with vascular cell adhesion molecule-1 expression; PODO: Podocyte; PEC: Parietal epithelial cells; PCT: Proximal convoluted tubule; PC: Principal cells; MES_FIB: Mesenchymal fibroblast; LEUK: Leukocytes; ICB: Type B intercalated cells; ICA: Type A intercalated cells; DCT: Distal convoluted tubule; CNT: Connecting tubule.