Bone marrow-derived mesenchymal stem cell-derived exosome-loaded miR-129-5p targets high-mobility group box 1 attenuates neurological-impairment after diabetic cerebral hemorrhage

Figure 10  Bone marrow-derived mesenchymal stem cell-derived exosome attenuated neuroinflammation after intracerebral hemorrhage in mice with diabetes by targeting the expression of high-mobility group box 1 in microglia through microRNA-129-5p. A: Quantitative polymerase chain reaction detected the expression levels of miR-129-5p and high-mobility group box 1 (HMGB1). The experimental data are expressed as mean ± SD. ^aP < 0.05, high glucose (HG) + hemin + miR-129-5p mimic negative control (NC) group vs HG + hemin + miR-129-5p mimic group; ^bP < 0.01, HG + hemin + miR-129-5p mimic group vs HG + hemin + miR-129-5p mimic + HMGB1 group, t-test; B: Quantitative polymerase chain reaction detected the expression levels of nuclear factor kappa B (NF-κB), interleukin (IL) 6, and toll-like receptor 4 inflammatory factors. The experimental data are expressed as mean ± SD. ^aP < 0.01, HG + hemin + miR-129-5p mimic NC group vs HG + hemin + miR-129-5p mimic group; ^bP < 0.001, HG + hemin + miR-129-5p mimic group vs HG + hemin + miR-129-5p mimic + HMGB1 group, t-test; C: Western blot detected the expression level of inflammatory factors, and the experimental data are expressed as mean ± SD. ^aP < 0.05, HG + hemin + miR-129-5p mimic NC group vs HG + hemin + miR-129-5p mimic group, t-test; D: Terminal deoxynucleotidyl transferase dUTP nick end labeling of neuronal apoptosis. The mimic NC group was HG + hemin + miR-129-5p mimic NC, the mimic group was HG + hemin + miR-129-5p mimic, and the mimic + HMGB1 group was HG + hemin + miR-129-5p mimic + HMGB1 in Panels B and D. HG: High glucose; NC: Negative control; HMGB1: High-mobility group box 1; TLR4: Toll-like receptor 4; IL: Interleukin; NF-κB: Nuclear factor kappa B; TNF: Tumor necrosis factor.