Bone marrow-derived mesenchymal stem cell-derived exosome-loaded miR-129-5p targets high-mobility group box 1 attenuates neurological-impairment after diabetic cerebral hemorrhage

Figure 8 Bone marrow-derived mesenchymal stem cell-derived exosome alleviated neuroinflammation and neurological impairment through microRNA-129-5p following intracerebral hemorrhage in mice with diabetes. A: The volume of intracerebral hemorrhage (ICH) detected by magnetic resonance imaging in mice. Experimental data are expressed as mean ± SD. ^aP < 0.05, ICH + diabetes mellitus (DM) group vs ICH + DM + bone marrow-derived mesenchymal stem cell-derived exosomes (BMSC-exo) group; ^aP < 0.05, ICH + DM + BMSC-exo group vs ICH + DM + miR-129-5p inhibitor group; ^aP < 0.05, ICH + DM + miR-129-5p inhibitor group vs ICH + DM + miR-129-5p inhibitor negative control (NC) group, t-test; B: Terminal deoxynucleotidyl transferase dUTP nick end labeling and Nissl staining were used to detect neuronal apoptosis and activity. Scale = 50 μm. Experimental data are expressed as mean ± SD. ^aP < 0.05, ICH + DM group vs ICH + DM + BMSC-exo group; ^aP < 0.05, ICH + DM + BMSC-exo group vs ICH + DM + miR-129-5p inhibitor group; ^aP < 0.05, ICH + DM + miR-129-5p inhibitor group vs ICH + DM + miR-129-5p inhibitor NC group, t-test; C: Blood-brain barrier injury was detected by Evans blue. Experimental data are expressed as mean ± SD. ^aP < 0.05, ICH + DM group vs ICH + DM + BMSC-exo group; ^aP < 0.05, ICH + DM + BMSC-exo group vs ICH + DM + miR-129-5p inhibitor group; ^aP < 0.05, ICH + DM + miR-129-5p inhibitor group vs ICH + DM + miR-129-5p inhibitor NC group, t-test; D: Nervous system score and balance beam test were used to detect the neurological function of the mice after ICH. The experimental data are expressed as mean ± SD. ^aP < 0.05, ICH + DM group vs ICH + DM + BMSC-exo group; ^aP < 0.05, ICH + DM + BMSC-exo group vs ICH + DM + miR-129-5p inhibitor group; ^aP < 0.05, ICH + DM + miR-129-5p inhibitor group vs ICH + DM + miR-129-5p inhibitor NC group, t-test. BMSC-exo: Bone marrow-derived mesenchymal stem cell-derived exosome; NC: Negative control; DM: Diabetes mellitus; ICH: Intracerebral hemorrhage.