Bone marrow-derived mesenchymal stem cell-derived exosome-loaded miR-129-5p targets high-mobility group box 1 attenuates neurological-impairment after diabetic cerebral hemorrhage

Figure 7 Bone marrow-derived mesenchymal stem cell-derived exosome overexpression of microRNA-129-5p attenuated neuro-inflammation after intracerebral hemorrhage in mice with diabetes. A: The expression of some inflammatory factors [toll-like receptor (TLR) 4, tumor necrosis factor (TNF)-α, high-mobility group box 1 (HMGB1), and interleukin (IL)-1β] in BV2 cells was detected by western blot. Experimental data are expressed as mean ± SD. ^aP < 0.05, high glucose (HG) + hemin + miR-129-5p mimic group vs HG + hemin + miR-129-5p mimic negative control (NC) group, t-test; ^bP < 0.01, HG + hemin group vs HG + hemin + miR-129-5p mimic group; B-D: Levels of inflammatory factors TLR4, IL-6, and nuclear factor kappa B (NF-κB) were detected by enzyme-linked immunosorbent assay. Experimental data are expressed as mean ± SD. ^aP < 0.05, intracerebral hemorrhage (ICH) + diabetes mellitus (DM) + miR-129-5p mimic group vs ICH + DM + miR-129-5p mimic NC group, t-test; ^bP < 0.01, ICH + DM group vs ICH + DM + miR-129-5p mimic group. The mimic group in panel A was HG + hemin + microRNA-129-5p mimic; the mimic NC group was HG + hemin + microRNA-129-5p mimic NC. TLR: Toll-like receptor; IL: Interleukin; TNF: Tumor necrosis factor; HMGB1: High-mobility group box 1; DM: Diabetes mellitus; ICH: Intracerebral hemorrhage; NF-κB: Nuclear factor kappa B; NC: Negative control.