Bone marrow-derived mesenchymal stem cell-derived exosome-loaded miR-129-5p targets high-mobility group box 1 attenuates neurological-impairment after diabetic cerebral hemorrhage

Figure 6 Overexpression of microRNA-129-5p attenuated neurological deficits after intracerebral hemorrhage in mice with diabetes. A: Brain sections and T2 magnetic resonance imaging to detect intracerebral hematoma volume in mice. Experimental data are expressed as mean ± SD. ^aP < 0.05, intracerebral hemorrhage (ICH) + diabetes mellitus (DM) + bone marrow-derived mesenchymal stem cell-derived exosomes (BMSC-exo) vs ICH + DM + miR-129-5p mimic group; ^aP < 0.05, ICH + DM + miR-129-5p mimic group vs ICH + DM + miR-129-5p mimic negative control (NC) group; ^bP < 0.01, ICH + DM group vs ICH + DM + BMSC-exo group; ^bP < 0.01, ICH + DM group vs ICH + DM + miR-129-5p mimic group, t-test; B: Immunofluorescence staining to detect the location of immunoglobulin G protein expression; C: Evans blue detection of blood-brain barrier damage. Experimental data are expressed as mean ± SD. ^aP < 0.05, ICH + DM + BMSC-exo group vs ICH + DM + miR-129-5p mimic group; ^aP < 0.05, ICH + DM + miR-129-5p mimic group vs ICH + DM + miR-129-5p mimic NC group; ^bP < 0.01, ICH + DM group vs ICH + DM + BMSC-exo group; ^bP < 0.01, ICH + DM group vs ICH + DM + miR-129-5p mimic group, t-test; D: Transmission electron microscopic observation of blood-brain barrier damage; E: Dry and wet weight method to measure edema after cerebral hemorrhage in mice. Experimental data are expressed as mean ± SD. ^aP < 0.05, ICH + DM + BMSC-exo group vs ICH + DM + miR-129-5p mimic group; ^aP < 0.05, ICH + DM + miR-129-5p mimic group vs ICH + DM + miR-129-5p mimic NC group; ^bP < 0.01, ICH + DM group vs ICH + DM + BMSC-exo group; ^bP < 0.01, ICH + DM group vs ICH + DM + miR-129-5p mimic group, t-test; F and G: Neurological score and balance beam test to detect the neurological function of mice after cerebral hemorrhage. Experimental data are expressed as mean ± SD. ^aP < 0.05, ICH + DM + BMSC-exo group vs ICH + DM + miR-129-5p mimic group; ^aP < 0.05, ICH + DM + miR-129-5p mimic group vs ICH + DM + miR-129-5p mimic NC group; ^bP < 0.01, ICH + DM group vs ICH + DM + BMSC-exo group; ^bP < 0.01, ICH + DM group vs ICH + DM + miR-129-5p mimic group, t-test. DM: Diabetes mellitus; ICH: Intracerebral hemorrhage; BMSC-exo: Bone marrow-derived mesenchymal stem cell-derived exosomes; NC: Negative control.