Corilagin alleviates podocyte injury in diabetic nephropathy by regulating autophagy via the SIRT1-AMPK pathway

Figure 9 Corilagin attenuates podocyte damage induced by hyperglycemia by enhancing autophagy. MPC5 cells were treated with the autophagy inhibitor 3-MA (1 mg/mL), corilagin (50 μM), and high glucose (D-glucose 30 mM) for 48 h. A: The effect of corilagin on cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; B: Cell apoptosis was assessed by Annexin V-FITC/propidium iodide double staining using flow cytometry; C: Representative images of flow cytometry. Data are presented as the mean ± SD. ^aP < 0.001 vs control group; ^bP < 0.001 vs HG group; ^cP < 0.001 vs HG + Cor group. HG: High glucose; Cor: Corilagin.