Functional analysis of the novel mitochondrial tRNATrp and tRNASer(AGY) variants associated with type 2 diabetes mellitus

doi:10.4239/wjd.v15.i8.1753

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World Journal of Diabetes

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1948-9358

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Diabetes. Aug 15, 2024; 15(8): 1753-1763
Published online Aug 15, 2024. doi: 10.4239/wjd.v15.i8.1753

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Figure 2 Analysis of steady-state levels of mitochondrial tRNA^Trp and mitochondrial tRNA^Ser(AGY) by Northern blotting. A: 2 µg mitochondrial tRNA (mt-RNA) were electrophoresed through a denaturing polyacrylamide gel and hybridized with DIG-labeled oligonucleotide probes for mt-tRNA^Trp, mt-tRNA^Ser(AGY), and 5S rRNA; B: Qualification of mt-tRNA levels.

Citation: Ding Y, Yu XJ, Guo QX, Leng JH. Functional analysis of the novel mitochondrial tRNA^Trp and tRNA^Ser(AGY) variants associated with type 2 diabetes mellitus. World J Diabetes 2024; 15(8): 1753-1763
URL: https://www.wjgnet.com/1948-9358/full/v15/i8/1753.htm
DOI: https://dx.doi.org/10.4239/wjd.v15.i8.1753