Interactions between myoblasts and macrophages under high glucose milieus result in inflammatory response and impaired insulin sensitivity

Figure 6 Inflammatory reaction induced by M1 macrophages impaired myotube insulin sensitivity. A: The GLUT4 fluorescence of myotube in the absence or presence of 100 nmo/L insulin by Immunofluorescence (scale bar: 50 μm); B: The 2-Deoxyglucose transport of myotube in the absence or presence of 100 nmo/L insulin by glucose uptake assay. Data are means ± SD, n = 6 per group. ^aP < 0.05, ^bP < 0.01 main effect of insulin; ^cP < 0.01 main effect of high glucose; ^dP < 0.01 main effect of co-cultured with macrophages. P values were calculated by two-way analysis of variances. M: Myoblasts; M + HG: Myoblasts cultured in high glucose medium; MMc: Myoblasts co-cultured with macrophages; MMc + HG: Myoblasts co-cultured with macrophages in high glucose medium; HG: High glucose.