Interactions between myoblasts and macrophages under high glucose milieus result in inflammatory response and impaired insulin sensitivity

Figure 5 Inflammatory reaction induced by M1 macrophages inhibited myogenic differentiation and led to myotube atrophy. A: Representative image of myotube by light microscopy, myotube were indicated with orange arrows (scale bar: 100 μm); B: The viability of myoblasts by CCK-8 assay; C: The ratios of E-MHC positive area in myotube by Immunofluorescence (scale bar: 50 μm). Data are means ± SD, n = 6 per group. ^aP < 0.01 main effect of high glucose; ^bP < 0.05, ^cP < 0.01 main effect of co-cultured with macrophages. P values were calculated by two-way analysis of variances. M: Myoblasts; M + HG: Myoblasts cultured in high glucose medium; MMc: Myoblasts co-cultured with macrophages; MMc + HG: Myoblasts co-cultured with macrophages in high glucose medium; HG: High glucose.