Interactions between myoblasts and macrophages under high glucose milieus result in inflammatory response and impaired insulin sensitivity

Figure 4 Interactions between myoblasts and macrophages under high glucose milieus resulted in chronic inflammation. The protein levels of monocyte chemoattractant protein 1, tumor necrosis factor-α, inerleukin-1β (IL-1β), IL-6 and IL-10 in culture medium by enzyme-linked immunosorbent assay. Data are means ± SD, n = 6 per group. ^aP < 0.01 main effect of high glucose; ^bP < 0.05, ^cP < 0.01 main effect of co-cultured with myoblasts. P values were calculated by two-way analysis of variances. Mc: Macrophages; Mc + HG: Macrophages cultured in high glucose medium; McM: Macrophages co-cultured with myoblasts; McM + HG: Macrophages co-cultured with myoblasts in high glucose medium; HG: High glucose.