Interactions between myoblasts and macrophages under high glucose milieus result in inflammatory response and impaired insulin sensitivity

Figure 3 Myoblast under high glucose milieus resulted in macrophages polarizing to pro-inflammatory M1 phenotype. A: The viability of macrophages by CCK-8 assay; B: Representative image of macrophages by Light Microscopy, inflammatory phenotype in morphology were indicated with orange arrows (Scale bar: 50 μm); C: The ratios of F4/80⁺ cells, F4/80⁺CD86⁺ cells, and F4/80⁺CD206⁺ cells in macrophages by Flow Cytometry. Data are means ± SD, n = 6 per group. ^aP < 0.01 main effect of high glucose; ^bP < 0.01 main effect of co-cultured with myoblasts. P values were calculated by two-way analysis of variances. Mc: Macrophages; Mc + HG: Macrophages cultured in high glucose medium; McM: Macrophages co-cultured with myoblasts; McM + HG: Macrophages co-cultured with myoblasts in high glucose medium; HG: High glucose.