Basic Study
Copyright ©The Author(s) 2024.
World J Diabetes. Jun 15, 2024; 15(6): 1340-1352
Published online Jun 15, 2024. doi: 10.4239/wjd.v15.i6.1340
Figure 4
Figure 4 The functions of growth hormone and adipocytokine after duodenal-jejunal bypass. A: Serum concentrations of growth hormone (GH), adiponectin, leptin and adipsin; B and C: Western blot (B) and reverse transcription-qPCR (RT-qPCR; C) analysis of GH receptor (GHR), adiponectin, leptin and adipsin in visceral adipose tissue; D: Oil red O staining of mature adipocytes; E: Phase microscope of primary visceral adipocyte; F and G: Western blot (F) and RT-qPCR (G) analysis of GHR, adiponectin, leptin and adipsin in mature adipocytes; H and I: Western blot (H) and RT-qPCR (I) analysis of GHR, adiponectin, leptin and adipsin in primary visceral adipocyte; J: Immunofluorescent staining of GLUT4 and INSR of mature adipocytes with or without Adiponectin knockdown; K: Average optical density of Immunofluorescent staining. aP < 0.05, bP < 0.01, cP < 0.001. GH: Growth hormone; GHR: Growth hormone receptor; PA: Palmitic acid; HG: High glucose; DJB: Duodenal-jejunal bypass; SAT: Subcutaneous adipose tissue; VAT: Visceral adipose tissue; AOD: Average optical density.