X-Paste improves wound healing in diabetes via NF-E2-related factor/HO-1 signaling pathway

Figure 4 Effect of Andro on proliferation and metastasis of human umbilical vein endothelial cells. A: The representative images of 5-ethynyl-2’-deoxyuridine, tubule formation and TUNEL in wound tissue sections from different groups; B: The wound healing assay in 0 h and 24 h from different groups; C: Immunofluorescence staining of CD31 and alpha-smooth muscle actin (α-SMA) in different groups; D and E: Western blot analysis of NF-E2-related factor-2 and HO-1 expression in each group and quantification of Western blot results; F and G: Western blot analysis of CD31, VEGFA, and α-SMA expression in each group and quantification of Western blot results; H and I: Western blot analysis of interleukin-6 and TNF-α expression in each group and quantification of Western blot results. Statistical significance is expressed as ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, P > 0.05 was not denoted. HG: High glucose; NC: Normal control; PBS: Phosphate-buffered saline; IL: Interleukin; TNF-α: Tumour necrosis factor alpha; Nrf2: NF-E2-related factor-2; Andro: Andrographolide.