Polycytosine RNA-binding protein 1 regulates osteoblast function via a ferroptosis pathway in type 2 diabetic osteoporosis

Figure 5 Polycytosine RNA-binding protein 1 regulation of osteoblastic ferroptosis in a high glucose environment. A: Western blotting results showing the expression levels of ferritin, glutathione peroxidase 4, osteoprotegerin, and osteocalcin after polycytosine RNA-binding protein 1 (PCBP1) lentivirus transfection in a high glucose (HG) environment; B: Flow cytometry results showing the levels of reactive oxygen species after PCBP1 lentivirus transfection in a HG environment; C: Alizarin red calcium nodule staining identified the number of calcium nodules in osteoblasts after PCBP1 lentivirus transfection in a HG environment. Data are presented as the mean ± SD. ^aP < 0.05 vs empty vector virus control; ^cP < 0.05 vs empty vector virus HG. PCBP1: Polycytosine RNA-binding protein 1; HG: High glucose; GPX4: Glutathione peroxidase 4; OPG: Osteoprotegerin; OCN: Osteocalcin.