Polycytosine RNA-binding protein 1 regulates osteoblast function via a ferroptosis pathway in type 2 diabetic osteoporosis

Figure 4 hFOB l. 19 cells were infected with polycytosine RNA-binding protein 1-encoded lentivirus, and the expression level of polycytosine RNA-binding protein 1 was detected via western blotting. A: Expression level of green fluorescently labeled PCBP1 in hFOB l. 19 cells was detected using an inverted fluorescence microscope; B: hFOB l. 19 cells were examined using a light microscope; C: Merging of A and B; D: Western blotting results of the protein expression level of polycytosine RNA-binding protein 1 in hFOB l. 19 cells. Data are presented as mean ± SD. ^aP < 0.05 vs control; ^cP < 0.05 vs empty vector virus control. Scale bar = 300 μm. PCBP1: Polycytosine RNA-binding protein 1.