Synaptotagmins family affect glucose transport in retinal pigment epithelial cells through their ubiquitination-mediated degradation and glucose transporter-1 regulation

Figure 6 The transcription factor myelin transcription factor 1 regulates synaptotagmins 4 expression. A: reverse transcriptase-polymerase chain reaction was used to measure the expression of myelin transcription factor 1 (MYT1); B: Western blot was used to detect the expression of MYT1 in cells; C: Western blot was used to determine the expression of MYT1 in mouse tissues; D: Western blot was used to determine the transfection efficiency of MYT1 in cells; E: The expression of synaptotagmins 4 was detected by Western blot. ^aP < 0.05, ^bP < 0.001, vs normal group, control group, NC group. DR: Diabetic retinopathy; MYT1: Myelin transcription factor 1.