Long-term effects of gestational diabetes mellitus on the pancreas of female mouse offspring

Figure 3 Adult female offspring born from dams with streptozotocin-induced gestational diabetes mellitus have a greater density of blood vessels vascularizing the acinar cells and the pancreatic islets. A, B, D and E: Representative confocal images of vehicle showing the endocrine and exocrine pancreatic tissue immunostained for endomucin blood vessels from adult progeny born from dams treated with vehicle or streptozotocin (STZ); C and F: There was a significantly greater density of blood vessels positive to endomucin in the acinar cells and the islets of Langerhans of offspring mice born from mice treated with STZ as compared to control mice; G and H: 3D visualization of the islet of Langerhans from whole pancreatic tissue processed with a solvent-based clearing method. Three-dimensional images of a cleared pancreas show an increased intra-islet vasculature. ^aP < 0.05 Student t test, n = 4-5 per group. Data are shown as mean ± SEM. VEH: Vehicle; STZ: Streptozotocin.