Long-term effects of gestational diabetes mellitus on the pancreas of female mouse offspring

Figure 2 Adult female offspring born from dams with streptozotocin-induced gestational diabetes mellitus have increased density of sensory and sympathetic nerve fibers innervating the pancreatic acinar cells. A and B: Representative confocal images of calcitonin gene-related peptide (CGRP) expressing small diameter peptidergic sensory nerve fibers; C and F: Significantly greater densities of CGRP⁺ and tyrosine hydroxylase⁺ (TH⁺) nerve fibers were found in offspring born from dams with GMD as compared to control offspring; D and E: Enzyme TH expressing postganglionic sympathetic nerve fibers in acinar pancreatic tissue (40 μm-thick) from offspring born of vehicle or streptozotocin injected dams; ^aP < 0.05 Student t test, n = 5 per group. Data are shown as mean ± SEM. CGRP: Calcitonin gene-related peptide; TH: Tyrosine hydroxylase; VEH: Vehicle; STZ: Streptozotocin.