Diabetes and high-glucose could upregulate the expression of receptor for activated C kinase 1 in retina

Figure 5 Silencing of receptor for activated C kinase 1 in adult retinal pigment epithelium cell line-19 cells under high glucose combined with hypoxia down-regulated protein kinase C-ε. A and B: The mRNA levels of receptor for activated C kinase 1 (RACK1) and protein kinase C-ε (PKC- ε) in the normal control, high glucose combined with hypoxia, non-silent siRNA, and silenced RACK1 siRNA groups; C-E: The protein levels of RACK1 and PKC-ε in adult retinal pigment epithelium cell line-19 cells of each group. The results are expressed as the mean ± SD. ^aP < 0.05 vs normal control. ^bP < 0.05 vs non-silent siRNA-normal control. NC: Normal control; Hg + Hypo: High glucose combined with hypoxia; siRNA-NC: Non-silent siRNA; siRNA-RACK1: Silenced RACK1 siRNA; RACK1: Receptor for activated C kinase 1; PKC- ε: Protein kinase C-ε.