MicroRNA-630 alleviates inflammatory reactions in rats with diabetic kidney disease by targeting toll-like receptor 4

Figure 4 Overexpression of miR-630 improves the biochemical changes in diabetic kidney disease model rats. A: Effects of miR-630 overexpression on body weight, renal weight index, blood glucose, fasting blood glucose, 24-h urinary protein, blood urea nitrogen and serum creatinine in rats; B: Effect of overexpression of miR-630 on the expression of toll-like receptor 4 (TLR4) mRNA in diabetic kidney disease rats; C: MiR-630 was overexpressed, and the levels of interleukin (IL)-6, IL-1β and tumor necrosis factor-α were detected by enzyme-linked immunosorbent assay; D: MiR-630 was overexpressed, and the expression levels of TLR4, E-cadherin, α-smooth muscle actin and collagen IV were detected by western blotting. Data are presented as mean ± SD, ^aP < 0.01 vs diabetic kidney disease + normal glucose agomir. DKD: Diabetic kidney disease; FBG: Fasting blood glucose; 24 h UP: 24-h urinary protein; BUN: Blood urea nitrogen; Scr: Serum creatinine; TLR4: Toll-like receptor 4; α-SMA: α-smooth muscle actin; IL: Interleukin; TNF: Tumor necrosis factor; NC agomir: Negative control of lentivirus.