Long noncoding RNA protein-disulfide isomerase-associated 3 regulated high glucose-induced podocyte apoptosis in diabetic nephropathy through targeting miR-139-3p

Figure 3 The direct interaction between long noncoding RNA Pdia3 and miR-139-3p was revealed. A: The subcellular location of long noncoding RNA (lncRNA) Pdia3 was examined by fluorescence in situ hybridization. LncRNA Pdia3 was mainly distributed in the cytoplasm; B: The target binding site between lncRNA Pdia3 and miR-139-3p was revealed; C: Dual-luciferase reporter assay was performed to measure the luciferase activity of co-transfecting with miR-139-3p mimics or NC mimics and lncRNA Pdia3 wt or mut luciferase reporters. The data were presented as the relative ratio of firefly luciferase activity to Renilla luciferase activity. ^aP < 0.05. ns: No significance. lncRNA: Long noncoding RNA; HG: High glucose; NG: Normal glucose; Pdia3: Protein-disulfide isomerase-associated 3; DAPI: 6-diamidino-2-phenylindole.