Shikonin protects mitochondria through the NFAT5/AMPK pathway for the treatment of diabetic wounds

Figure 4 Regulation of NFAT5 and AMPK pathways in hypertonic cells: Protein and mRNA expression, and effects on cell viability. A: The expressions of NFAT5 protein in hypertonic cells; B: The expressions of protein (NFAT5, AMPK) with NFAT5 overexpression vector and siNFAT5 transfection into hypertonic cells were detected; C: The expressions of mRNA (NFAT5, AMPK) with NFAT5 overexpression vector and siNFAT5 transfection into hypertonic cells were detected; D: The expression of AMPK in hypertonic cells was detected; E: The effects of NFAT5 and AMPK inhibitors on cell viability were detected. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.