Shikonin protects mitochondria through the NFAT5/AMPK pathway for the treatment of diabetic wounds

Figure 2 Assessment of mitochondrial ultrastructure, reactive oxygen species, and membrane potential in NG, MG, HG, and SHG groups. A: Changes of mitochondria under transmission electron microscope. Mitochondrial ultrastructure was identified in NG MG, HG, SHG group. Mitochondria marked with red arrows; B: The fluorescence expression of reactive oxygen species: NG, MG, HG, SHG group; C: The fluorescence expression of mitochondrial membrane potential tested by JC-1: NG, MG, HG, SHG group; D: The fluorescence expression of mitochondrial membrane potential tested by calcein AM: NG, MG, HG, SHG group.