Effect of exercise during pregnancy on offspring development through ameliorating high glucose and hypoxia in gestational diabetes mellitus

Figure 3 High glucose and hypoxia inhibit the migration capacity of HTR8/SVneo cells. A: Western blot analysis results of HIF-1a expression in HTR8/SVneo cells after treatment with 200 μM and 400 μM CoCl₂; B and D: Migration capacity of HTR8/SVneo cells analyzed by Transwell assay; C and E: Wound-healing assay. Data are presented as the mean ± SEM. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. GLUT: Glucose transporter; HIF-1α: Hypoxia inducible factor-1α; CoCl₂: Cobalt chloride; ACTB: β-Actin; PBS: Phosphate buffered saline.