Effect of exercise during pregnancy on offspring development through ameliorating high glucose and hypoxia in gestational diabetes mellitus

Figure 2 Downregulation of glucose transporter 1 inhibits the glucose intake capability of high glucose-treated HTR8/SVneo cells. A and B: mRNA and protein expression of glucose transporter 1 in high glucose-treated transfected HTR8/SVneo cells were detected by reverse-transcriptase polymerase chain reaction and western blotting; C and D: Glucose intake capacity of HTR8/SVneo cells was analyzed using flow cytometry. Data are presented as the mean ± SEM. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. GLUT: Glucose transporter; NC: Negative control; 2-NBDG: 2-Deoxy-2- [(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose; ACTB: β-Actin.