Asiaticoside improves diabetic nephropathy by reducing inflammation, oxidative stress, and fibrosis: An in vitro and in vivo study

Figure 3 Effect of asiaticoside on high glucose-induced fibrosis in rat glomerular mesangial cells. A:Quantitative real-time polymerase chain reaction analysis of the mRNA levels of collagen I, collagen IV, and fibronectin in rat glomerular mesangial (HBZY-1) cells treated with asiaticoside at different concentrations (0, 2, 4, and 8 μM) and glucose (30 mmol/L); B: Western blot analysis of the protein levels of collagen I, collagen IV, and fibronectin in HBZY-1 cells. n = 3 experiments. ^aP < 0.001 vs control; ^bP < 0.05, ^cP < 0.01, and ^dP < 0.001 vs Glu (30 mmol/L). AC: Asiaticoside; Glu: Glucose.