Identification of immune feature genes and intercellular profiles in diabetic cardiomyopathy

Figure 7 Analysis of the expression patterns of immune feature genes and cell-cell communication. A: UMAP dimensionality reduction embedding was performed on the integrated dataset of single-cell RNA sequencing data from all profiled samples (n = 12593 cells). The embedding is color-coded by inferred cluster identity and annotated with manual cell type labels; B: Clusters with similar marker gene expression modules were merged and clustered into the same cell type; C: Dot plot showing cell-specific markers; D: Violin plots showing statistically significant feature gene (proenkephalin and retinol binding protein 7) expression values for each cell type, grouped by the donor of origin [control (CTL) and diabetic cardiomyopathy (DCM)]; E: UMAP for cell clusters grouped by the donor of origin (CTL or DCM); F: Bar chart representing the cell counts of different cell types in the two groups. DCM: Diabetic cardiomyopathy; CTL: Control; Penk: Proenkephalin; Gcgr: Glucagon receptor; Rbp7: Retinol binding protein 7; Inha: Inhibin subunit alpha.