Network-pharmacology-based research on protective effects and underlying mechanism of Shuxin decoction against myocardial ischemia/reperfusion injury with diabetes

Figure 11  Shuxin decoction attenuates myocardial apoptosis in diabetic rats with myocardial ischemia/reperfusion injury. A: TUNEL staining. TUNEL-positive nuclei are stained green, while nuclei of cardiomyocytes are blue; B: Percentage of positive apoptosis cardiomyocyte (green/blue fluorescence, magnification × 20, scale bars, 50 μM); C: Bcl-2, Bax, and cleaved caspase-3 protein levels detected by Western blot; D: Statistics of gray value of Bcl-2/GAPDH based on Western blot; E: Statistics of gray value of Bax/GAPDH based on Western blot; F: Statistics of gray value of cleaved caspase-3/GAPDH based on Western blot. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs group C. ^dP < 0.05, ^eP < 0.01, ^fP < 0.001 vs myocardial ischemia/reperfusion injury in diabetes group (n = 3 rats per group). C: Normal control group; DS: Diabetic rats with sham operation group; MI/RI: Myocardial ischemia/reperfusion injury; DMR: MI/RI in diabetes group; SXTL: MI/RI in diabetic rats receiving SXT 0.7 g/kg/d group; SXTM: MI/RI in diabetic rats receiving SXT 1.4 g/kg/d group; SXTH: MI/RI in diabetic rats receiving SXT 2.8 g/kg/d group.