Glucose metabolism continuous deteriorating in male patients with human immunodeficiency virus accepted antiretroviral therapy for 156 weeks

Figure 1 Schematic diagram. A total of 61 cases were included, including 50 cases with normal glucose tolerance, 11 cases with prediabetes, and none of them with diabetes mellitus. All cases were treated with the efavirenz plus lamivudine plus tenofovir regimen for 156 wk. At 0, 4, 8, 12, 24, 36, 48, 60, 72, 84, 96, 108, 120, 132, 144, and 156 wk, venous blood was drawn from the patients who fasted overnight for at least 12 h for fasting plasma glucose using the glucose oxidase method by the semiautomatic Dias STAT Model 550 analyzer (Bio-Rad) with the kit purchased from Zhejiang Eastern European Biological Products Company; at 0, 12, 24, 36, 48, 72, 96, 120, and 144 wk for fasting serum insulin using electrochemiluminescence immunoassay by automatic Elecsys2010 analyzer (Roche); and for glycosylated hemoglobin using high-pressure liquid chromatography by glycosylated hemoglobin (G7) analyzer (Tosoh Company) with a kit purchased from Bio-Rad in the central laboratory.