Tongxinluo promotes endothelium-dependent arteriogenesis to attenuate diabetic peripheral arterial disease

Figure 8 Tongxinluo enhanced the interaction between human vascular endothelial cells and vascular smooth muscle cells (VSMCs) and promoted the migration of VSMCs. A and B: Schematic diagram and photos of the coculture system; C-E: Quantitative analysis of Jagged-1, Ntoch-1 and ephrin-B2 mRNA expression was determined via RT-PCR; F: Scratch assay to detect VSMC migration ability. G: Percentage of the wound healing area was measured using ImageJ software; H: Transwell assays were performed to detect VSMC migration ability and the relative cell migration number was calculated by ImageJ software. The values are shown as the means ± SDs. ^aP < 0.05 compared with the normal group, ^bP <0.05 compared with the model group, ^cP < 0.05 compared with the TXL-L group, n = 3. TXL: Tongxinluo; HUVECs: Human vascular endothelial cells; VSMCs: Vascular smooth muscle cells.