Tongxinluo promotes endothelium-dependent arteriogenesis to attenuate diabetic peripheral arterial disease

Figure 7 Tongxinluo promoted human vascular endothelial cell (HUVEC) proliferation, restored HUVEC function, and inhibited pyroptosis. A: Human vascular endothelial cell (HUVEC) morphology; B: Cell counting Kit-8 assay for HUVEC proliferation rate; C: NO kit to detect NO levels in in HUVEC supernatants; D-G: The expression of the endothelial-associated proteins endothelial nitric oxide synthase, vascular endothelial growth factor, VE-cadherin and β-catenin in the HUVEC supernatants was analyzed by RT-PCR; H: HUVECs for tubule formation experiments; I: Flow cytometry detection of cell pyroptosis; J: Statistical analysis of the percentage of caspase-1 and PI double-stained positive cells in the total cells. The values are shown as the means ± SDs. ^aP < 0.05 compared with the normal group, ^bP <0.05 compared with the model group, ^cP < 0.05 compared with the TXL-L group, n = 3. TXL: Tongxinluo; VEGF: Vascular endothelial growth factor; eNOS: Endothelial nitric oxide synthase.