miR-124 is upregulated in diabetic mice and inhibits proliferation and promotes apoptosis of high-glucose-induced β-cells by targeting EZH2

Figure 2 miR-124 inhibitor promoted proliferation and the transition from the G0/G1 phase to the S phase of the cell cycle, suppressed the apoptosis and promoted insulin secretion in high glucose-induced Min6 cells. A: The relative expression of miR-124 was upregulated in high glucose (HG)-induced Min6 cells, and was down-regulated by miR-124 inhibitor detected by real-time quantitative polymerase chain reaction; B: miR-124 inhibitor promoted the proliferation ability of HG-induced Min6 cells; C: miR-124 inhibitor promoted the HG-induced Min6 cells at the transition from the G0/G1 phase to the S phase of the cell cycle; D: miR-124 inhibitor suppressed the apoptosis of HG-induced Min6 cells detected by flow cytometry; E: Relative expression of apoptotic related proteins Bcl-2, Bax and caspase-3 were detected by western blot; F: miR-124 inhibitor increased the secretion of insulin in HG-induced Min6 cells. ^aP < 0.05, vs BC group; ^bP < 0.05, vs the HG group. BC: Blank control; HG: High glucose.