Depletion of gut microbiota facilitates fibroblast growth factor 21-mediated protection against acute pancreatitis in diabetic mice

Figure 2 Fibroblast growth factor 21 treatment mitigates intestinal damage and inflammation in diabetic mice with acute pancreatitis. A: Histological changes in the small intestine of mice in diabetic, acute pancreatitis and fibroblast growth factor 21 groups, characterized by tissue edema, increased villus width, and villus damage (scale bar: 100 µm); B-E: Representative immunoblots of inflammatory factors in mouse small intestinal tissue. Expression levels of interleukin-6 and tumor necrosis factor-alpha were quantified using densitometry, with GAPDH as a protein loading control. Data are presented as mean ± SD, ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. AP: Acute pancreatitis; db: Diabetic; IL: Interleukin; TNF-: Tumor necrosis factor-alpha; FGF21: Fibroblast growth factor 21.