Depletion of gut microbiota facilitates fibroblast growth factor 21-mediated protection against acute pancreatitis in diabetic mice

Figure 1 Fibroblast growth factor 21 significantly attenuates pancreatic injury and inflammation in diabetic mice with acute pancreatitis. A: The ratio of pancreas weight to body weight of mice in diabetic (db), acute pancreatitis (AP) and fibroblast growth factor 21 (FGF21) groups; B: Serum levels of amylase of mice in db, AP and FGF21 groups; C-E: Representative immunoblots of inflammatory factors in mouse pancreatic tissue. Expression levels of interleukin-6 and tumor necrosis factor-alpha were quantified using densitometry, with GAPDH as a protein loading control; F: Pathological changes in pancreatic tissues of mice in db, AP and FGF21 groups, such as pancreatic edema, extensive intracellular vacuolation, and cellular necrosis (scale bar: 100 µm). Data are presented as mean ± SD, ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. AP: Acute pancreatitis; AMS: Amylase; db: Diabetic; IL: Interleukin; TNF-: Tumor necrosis factor-alpha; FGF21: Fibroblast growth factor 21.