Atorvastatin ameliorated myocardial fibrosis in db/db mice by inhibiting oxidative stress and modulating macrophage polarization

Table 1 Primers used in this study

Gene	Primer	Tm (°C)	Product length	Accession
TGF-β1	Forward: 5’-CTCCCGTGGCTTCTAGTGC-3’	60.15	133	NM_011577.2
	Reverse: 5’-GCCTTAGTTTGGACAGGATCTG-3’	58.73
TNF-α	Forward: 5’-CCCTCACACTCAGATCATCTTCT-3’	59.29	61	NM_013693.3
	Reverse: 5’-GCTACGACGTGGGCTACAG-3’	60.23
IL-1β	Forward: 5’-GCAACTGTTCCTGAACTCAACT-3’	59.05	89	NM_008361.4
	Reverse: 5’-ATCTTTTGGGGTCCGTCAACT-3’	59.58
18S rRNA	Forward: 5’-AGGGGAGAGCGGGTAAGAGA-3’	61.58	241	AH002077.2
	Reverse: 5’-GGACAGGACTAGGCGGAACA-3’	61.26

Tm: Melting temperature; TGF: Transforming growth factor; TNF: Tumor necrosis factor; IL: Interleukin.