Vascular endothelial growth factor B improves impaired glucose tolerance through insulin-mediated inhibition of glucagon secretion

Figure 1 The construction of impaired glucose tolerance mice with vascular endothelial growth factor B gene knockout and overexpressed. A: Active Stain of the islet cell mass in high-fat diet (HFD)-WT and HFD-KO mice; B: The mRNA expression of vascular endothelial growth factor B (VEGFB) in the islet of mice in the HFD-WT and HFD-KO group; C: The ingredients of standard diet (SD) and HFD; D: Green fluorescence staining of the islet with adeno-associated virus (AAV) infection in Con and AAV mice; E: The mRNA expression of VEGFB in the islet of mice in the Con and AAV group; F: The flow chart of animal experiments; G: Fasting blood glucose at the 16^th wk; H: PBG at the 16^th wk; I: Oral glucose tolerance test at the 16^th wk; J: Area under the curve at the 16^th wk. ^aP < 0.05 vs vegfb^+/+; ^aP < 0.05 vs Con; ^aP < 0.05 vs SD. HFD: High-fat diet; AAV: Adeno-associated virus; SD: Standard diet; VEGF: Vascular endothelial growth factor.