Copyright
©The Author(s) 2023.
World J Diabetes. Nov 15, 2023; 14(11): 1672-1692
Published online Nov 15, 2023. doi: 10.4239/wjd.v14.i11.1672
Published online Nov 15, 2023. doi: 10.4239/wjd.v14.i11.1672
Reagent | Manufacturer | Article number |
DMEM high sugar medium | Gibco Corporation, United States | 11960044 |
Fetal bovine serum | Corning Corporation, United States | 35-081-CV |
Phosphate buffer | Beijing Soleibao Company | SH30256.01 |
0.25% trypsin protein | Gibco Corporation, United States | T1320 |
Green streptomycin mixture | Beijing Soleibao Company | P1400-100 |
CCK-8 kit | Jiangsu Kaiji Biology | KGA317 |
Cell cycle kit | Jiangsu Kaiji Biology | KGA512 |
Apoptosis kit | Jiangsu Kaiji Biology | KGA101 |
BCA protein content detection kit | Jiangsu Kaiji Biology | KGP902 |
Total protein extraction kit | Jiangsu Kaiji Biology | KGP250 |
5× loading buffer | Jiangsu Kaiji Biology | KGP101 |
Chemiluminescence kit | Jiangsu Kaiji Biology | KGS133 |
10× WB washing solution | Jiangsu Kaiji Biology | KGP109 |
PVDF membrane | Millipore Corporation | IPVH00010 |
10× electrotransfer buffer | Jiangsu Kaiji Biology | KGP102 |
0.25% trypsin protein (EDTA not included) | Gibco Corporation | T1320 |
PI3K rabbit antibody | Abcam | ab302958 |
p-PI3K rabbit antibody | Abcam | ab278545 |
AKT rabbit antibody | Abcam | ab283852 |
Rabbit antibody to p-AKT | Abcam | ab38449 |
INSR Rabbit antibody | Wuhan Sanying | 20433-1-AP |
Rabbit antibody to GSK3 | Wuhan Sanying | 13419-1-AP |
Rabbit IgG | Santa Cruzgong Company, United States | sc2004 |
GAPDH | Abcam | Ab8245 |
- Citation: Meng FD, Yuan L, Xu DJ, Che MY, Hou SZ, Lu DD, Liu WJ, Nan Y. Exploring the targets and molecular mechanism of glycyrrhetinic acid against diabetic nephropathy based on network pharmacology and molecular docking. World J Diabetes 2023; 14(11): 1672-1692
- URL: https://www.wjgnet.com/1948-9358/full/v14/i11/1672.htm
- DOI: https://dx.doi.org/10.4239/wjd.v14.i11.1672