Exploring the targets and molecular mechanism of glycyrrhetinic acid against diabetic nephropathy based on network pharmacology and molecular docking

doi:10.4239/wjd.v14.i11.1672

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World Journal of Diabetes

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1948-9358

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Diabetes. Nov 15, 2023; 14(11): 1672-1692
Published online Nov 15, 2023. doi: 10.4239/wjd.v14.i11.1672

Table 1 Drugs and reagents

Reagent	Manufacturer	Article number
DMEM high sugar medium	Gibco Corporation, United States	11960044
Fetal bovine serum	Corning Corporation, United States	35-081-CV
Phosphate buffer	Beijing Soleibao Company	SH30256.01
0.25% trypsin protein	Gibco Corporation, United States	T1320
Green streptomycin mixture	Beijing Soleibao Company	P1400-100
CCK-8 kit	Jiangsu Kaiji Biology	KGA317
Cell cycle kit	Jiangsu Kaiji Biology	KGA512
Apoptosis kit	Jiangsu Kaiji Biology	KGA101
BCA protein content detection kit	Jiangsu Kaiji Biology	KGP902
Total protein extraction kit	Jiangsu Kaiji Biology	KGP250
5× loading buffer	Jiangsu Kaiji Biology	KGP101
Chemiluminescence kit	Jiangsu Kaiji Biology	KGS133
10× WB washing solution	Jiangsu Kaiji Biology	KGP109
PVDF membrane	Millipore Corporation	IPVH00010
10× electrotransfer buffer	Jiangsu Kaiji Biology	KGP102
0.25% trypsin protein (EDTA not included)	Gibco Corporation	T1320
PI3K rabbit antibody	Abcam	ab302958
p-PI3K rabbit antibody	Abcam	ab278545
AKT rabbit antibody	Abcam	ab283852
Rabbit antibody to p-AKT	Abcam	ab38449
INSR Rabbit antibody	Wuhan Sanying	20433-1-AP
Rabbit antibody to GSK3	Wuhan Sanying	13419-1-AP
Rabbit IgG	Santa Cruzgong Company, United States	sc2004
GAPDH	Abcam	Ab8245

Citation: Meng FD, Yuan L, Xu DJ, Che MY, Hou SZ, Lu DD, Liu WJ, Nan Y. Exploring the targets and molecular mechanism of glycyrrhetinic acid against diabetic nephropathy based on network pharmacology and molecular docking. World J Diabetes 2023; 14(11): 1672-1692
URL: https://www.wjgnet.com/1948-9358/full/v14/i11/1672.htm
DOI: https://dx.doi.org/10.4239/wjd.v14.i11.1672