Basic Study
Copyright ©The Author(s) 2023.
World J Diabetes. Nov 15, 2023; 14(11): 1672-1692
Published online Nov 15, 2023. doi: 10.4239/wjd.v14.i11.1672
Table 1 Drugs and reagents
Reagent
Manufacturer
Article number
DMEM high sugar mediumGibco Corporation, United States11960044
Fetal bovine serumCorning Corporation, United States35-081-CV
Phosphate bufferBeijing Soleibao CompanySH30256.01
0.25% trypsin proteinGibco Corporation, United StatesT1320
Green streptomycin mixtureBeijing Soleibao CompanyP1400-100
CCK-8 kitJiangsu Kaiji BiologyKGA317
Cell cycle kitJiangsu Kaiji BiologyKGA512
Apoptosis kitJiangsu Kaiji BiologyKGA101
BCA protein content detection kitJiangsu Kaiji BiologyKGP902
Total protein extraction kitJiangsu Kaiji BiologyKGP250
5× loading bufferJiangsu Kaiji BiologyKGP101
Chemiluminescence kitJiangsu Kaiji BiologyKGS133
10× WB washing solutionJiangsu Kaiji BiologyKGP109
PVDF membraneMillipore CorporationIPVH00010
10× electrotransfer bufferJiangsu Kaiji BiologyKGP102
0.25% trypsin protein (EDTA not included)Gibco CorporationT1320
PI3K rabbit antibodyAbcamab302958
p-PI3K rabbit antibodyAbcamab278545
AKT rabbit antibodyAbcamab283852
Rabbit antibody to p-AKTAbcamab38449
INSR Rabbit antibodyWuhan Sanying20433-1-AP
Rabbit antibody to GSK3Wuhan Sanying13419-1-AP
Rabbit IgGSanta Cruzgong Company, United Statessc2004
GAPDHAbcamAb8245