Advances in microfluidic chips based on islet hormone-sensing techniques

Figure 3 Localized surface plasmon resonance-based islet secretome sensing. A: Theory of localized surface plasmon resonance (LSPR) and spectral curve before and after antigen binding; B: A real-world system for online islet trapping, perfusion and islet secretome monitoring. In general, the perfusate is pumped into the islet trapping chip by the peristaltic pump, and islet secretions are pumped out to the LSPR chip for further measurement; a: Culture medium or perfusate for islets; b: Peristaltic pump provide force for buffer; c: Islet trapping chip with tissue culture module; d: The LSPR detection module for spectral shift signal capturing; e: Work station for real-time signal transducing. LSPR: Localized surface plasmon resonance. A: Citation: Frederix F, Friedt JM, Choi KH, Laureyn W, Campitelli A, Mondelaers D, Maes G, Borghs G. Biosensing based on light absorption of nanoscaled gold and silver particles. Anal Chem 2003; 75: 6894-6900. Copyright© American Chemical Society 2003. Published by ACS Publications. The authors have obtained the permission for figure using from the American Chemical Society (Supplementary material); B: Citation: Ortega MA, Rodríguez-Comas J, Yavas O, Velasco-Mallorquí F, Balaguer-Trias J, Parra V, Novials A, Servitja JM, Quidant R, Ramón-Azcón J. In Situ LSPR Sensing of Secreted Insulin in Organ-on-Chip. Biosensors (Basel) 2021; 11. Copyright© 2021 by the authors. Published by MDPI. No special permission is required to reuse all or part of article published by MDPI, including figures and tables (https://www.mdpi.com/openaccess).