Hyperglycemia and reduced adiposity of streptozotocin-induced diabetic mice are not alleviated by oral benzylamine supplementation

Figure 4 Direct stimulation by insulin and Bza of hexose uptake in adipocytes: lack of influence of Bza supplementation and invitro inhibition by phenelzine. A: Radiolabeled 2-deoxyglucose (2-DG) uptake was determined in basal condition or in response to increasing doses of insulin in adipocytes from nondiabetic mice of the water-drinking (open circles) or Bza-drinking (red circles) group, while it could not be determined in diabetic mice due to the scarcity of adipocytes isolated from their emaciated fat depots, in both control and Bza-drinking groups. Mean ± SEM of eight adipocyte preparations. B: 2-DG uptake was determined after 45 min incubation without (basal) or with 200 nmol/L insulin and 0.1 mmol/L Bza. The stimulated hexose uptake was significantly different from basal at: ^aP < 0.001; ^bP < 0.01. Phenelzine was added at 0.1 mmol/L in the incubation medium of adipocytes from control nondiabetic mice (blue columns) or from Bza-drinking nondiabetic mice (purple columns). Phenelzine inhibited significantly Bza-induced hexose uptake at: ^cP < 0.01. STZ: Streptozotocin; Bza: Benzylamine; WAT: White adipose tissues.