Long noncoding RNA X-inactive specific transcript regulates NLR family pyrin domain containing 3/caspase-1-mediated pyroptosis in diabetic nephropathy

doi:10.4239/wjd.v13.i4.358

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Apr 15, 2022 (publication date) through Nov 4, 2024

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World Journal of Diabetes

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World J Diabetes. Apr 15, 2022; 13(4): 358-375
Published online Apr 15, 2022. doi: 10.4239/wjd.v13.i4.358

Table 1 Primer sequences

	Forward Primer (5′-3′)	Reverse Primer (5′-3′)
XIST (human)	AGCTCCTCGGACAGCTCTAA	CTCCAGATAGCTGGCAACC
XIST (Norway rat)	CCCATACCCTCATACCCTAATG	GGCTGGCCTCATTTCTGGGCTC
hsa-miR-15b-5p	ATCCAGTGCGTGTCGTG	TGCTTAGCAGCACATCATG
rno-miR-15b-5p	GCCGAGTAGCAGCACATCATG	CTCAACTGGTGTCGTGGA
hsa-pre-miR-15b	GGCCTTAAAGTACTGTAGCAGC	CTCAACTGGTGTCGTGGA
rno-pre-miR-15b	GGAGTTTTTCCCTTTTGGATG	ATAATGATTCGCATCTTGATTGTAG
TLR4 (human)	CCGCTCTGGCATCATCTTCA	TGGGTTTTAGGCGCAGAGTT
TLR4 (Norway rat)	GCCAGGATGATGCCTCTCTTGC	CCTCAGGTCAAAGTTGTTGC
U6 (human)	GCTCGCTTCGGCAGCACA	GAGGTATTCGCACCAGAGGA
U6 (Norway rat)	ATGGCGGACGACGTAGATCAGCA	TCAGCCAACTCTCAATGGAGGGG
GAPDH (human)	TCCATGACAACTTTGGCATC	CATGTCAGATCCACCACGGA
GAPDH (Norway rat)	CAAGATGGTGAAGGTCGGTGT	CTTACTCCTTGGAGGCCATGTAG

XIST: X inactive specific transcript; TLR4: toll like receptor 4; hsa: Homo sapiens; rno: Rattus norvegicus; U6: U6 small nuclear RNA; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; pre: precursor; miR: microRNA.

Citation: Xu J, Wang Q, Song YF, Xu XH, Zhu H, Chen PD, Ren YP. Long noncoding RNA X-inactive specific transcript regulates NLR family pyrin domain containing 3/caspase-1-mediated pyroptosis in diabetic nephropathy. World J Diabetes 2022; 13(4): 358-375
URL: https://www.wjgnet.com/1948-9358/full/v13/i4/358.htm
DOI: https://dx.doi.org/10.4239/wjd.v13.i4.358