High doses of catecholamines activate glucose transport in human adipocytes independently from adrenoceptor stimulation or vanadium addition

Figure 7 Inhibition of adrenaline-induced hexose uptake in human adipocytes by catalase, glutathione and wortmannin. The pretreatment of adipocytes by catalase alone (5000 IU/mL) or in combination with 1 mmol/L glutathione was started 10 min before the addition of 1 mmol/L adrenaline, which was incubated for 45 min before performing 2-deoxyglucose uptake assay for 10 min. The mean ± standard error of the mean of 4 to 6 separate experiments is given as fold increase above basal 2-deoxyglucose uptake. A significant difference from basal was observed at: ^cP < 0.001; Different from 1 mmol/L adrenaline alone at: ^aP < 0.05, or from 100 nmol/L insulin alone at: ^bP < 0.001. 2-DG: 2-Deoxyglucose; adre: Adrenaline.