Decarboxylated osteocalcin, a possible drug for type 2 diabetes, triggers glucose uptake in MG63 cells

Figure 2 Flow cytometric analyses of 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose uptake by MG63 cells after decarboxylated osteocalcin stimulation. A: The distribution of fluorescence intensities which represented 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose (2-NBDG) uptake after decarboxylated osteocalcin (dcOC) stimulation for 1 h; B: The normalized fluorescence intensities corresponding to 2-NBDG uptake after dcOC stimulation for 1 h; C: Distribution of fluorescence intensities which represented 2-NBDG uptake after dcOC stimulation for 72 h; D: The normalized fluorescence intensity corresponding to 2-NBDG uptake after dcOC stimulation for 72 h. The red lines in the histograms in A and C indicate the control group (0 ng/mL), while the other lines indicate the groups treated with dcOC at different concentrations. The data are representative of three independent experiments performed in triplicate. ^aP < 0.05, ^bP < 0.01 vs the control group.